Sodium citrate buffer inoshandiswa kuRNA kusungirirwa nokuti inoderedza chidimbu che hydrolysis yeRNA michina. Izvi zvinoita kuti zvive nhanho inokosha mu mRNA kucheneswa munguva yekutsvakurudza kwema genomic kana yekudzidza kunyorwa.
Zvinyorwa zvinogadziriswa zvinogona kubatsirawo kuonekwa kweantigens muzvigadziriswe zvigadziriswe zvigadziriswa nokuti zvinoputsa mitsetse-miviri yakaumbwa pakati peantigens uye mharidzo yekugadzirisa.
Uine motungamiri pasi apa, ita sodium citrate buffer ye pH 6. Mirayiridzo haisi nyore chete asi inokurumidza kuita zvakare. Inofanira kukutora iwe maminitsi gumi kuti upedze matanho aya.
Zvisarudzo zviviri Kuti Uite Bhuku
Pane nzira mbiri dzekuita nezvekuita sodium citrate buffer. Chokutanga, sarudza kana iwe une zvose citric acid uye svondo re conjugate, sodium citrate, kushanda pamwe chete. Kana iwe chete uine citric acid, haisi dambudziko. Iwe unogona kugadzirisa chikwata.
Kune rumwe rutivi, kana iwe une zvose asiri uye zvigadziriswa, ita chigadziriswa chekuchengetedza kweumwe neumwe. Sakanisa magiramu 21 e citric acid mu 1 litre yemvura yakasvibiswa, uye 29.4 gramu ye sodium citrate mu 1 litre yemvura yakasvibiswa.
Kana iwe uine citric acid chete, shandisa 2.1 gramu mumvura iri pasi pe 1 litre yemvura yakasvibiswa uye enda kune imwe danho rinotevera.
Kusanganisa Citric Acid uye Sodium Citrate Solutions
Iye zvino zvawakasimbisa soro yako (kana iwe wakanga uine asidi uye nheyo) kana ukavhenganisa asidi yako mumvura yakasvibiswa (kana iwe usina), unogona kutarisa pane zvaunogadzirisa.
Sanisa 82 milliliters e citric acid solution nemamiriyoni 18 e sodium citrate solution.
Zvadaro, ita uwandu hwehuwandu hwese kusvika kune 1 litre nemvura yakasvibiswa. Siyai zvakakwana kuvhura kuti kuwedzerwa kwe-sodium hydroxide (NaOH) muchikamu chinotevera.
Kugadzirisa pH
Paunenge uchifambisa zvinyoro zvinyorwa uchishandisa maginist stirrer, shandisa 1M sodium hydroxide kuti uchinje pH yesanganiswa kusvika 6.0. Mushure mokudaro, ita gwaro rekupedzisira remubhedha kusvika 1 litre nemvura yakasvibiswa nekushandisa bundu rinopisa.
Zvaunoda Kuti Uite
Kwete chete hazvizokutora iwe kwenguva yakareba kuti uite sodium citrate buffer, iwewo haungatengi zvinhu zvakawanda zvazviri. Uine citric acid, 1M ye sodium hydroxide, mvura yakasvibiswa, uye purogiramu yepH yakatarwa, iwe uchava pakati payo. Kushandisa sodium citrate kunosarudza.
Kuita mutsara kunodawo 1 litre kupedza cylinder, 1-litre volumetric flask, uye zvitatu 1 litre mabhodhoro emidziyo. Pakupedzisira, iwe uchada magnetic stir bar uye magnetic stirrer.
Kana iwe usina zvinhu zvose izvi, funga kushandisa kirairi pachikoro chako kana nzvimbo yebasa, naizvozvo haufaniri kuzvitenga iwe pachako. Iwe unogonawo kutenga zviri nyore zvinhu izvi muIndaneti kana kushanyira imwe nzvimbo yekutengesa zvivako munzvimbo yako.