Nzira Yokuita Nayo Immunohistochemical Staining Buffer
Phosphate buffered saline (PBS) inogadzirisa mishonga iyo inowanzoshandiswa pakugadzira immunohistochemical (IHC) uye inowanzoshandiswa mukutsvakurudza kwehupenyu. PBS inogadziriswa nemvura yakagadzirwa nemunyu ine sodium hydrogen phosphate, sodium chloride uye, mune dzimwe nguva, potassium chloride uye potassium dihydrogen phosphate.
Immunohistochemical Staining
Immunohistochemistry inoreva nzira yekucherechedza maantijeni akadai semaprotineni mumasero echikamu chechidimbu achishandisa nhungamiro yezvirwere zvinosunga zvakananga kune antigens muzvigadzirwa zvehupenyu.
Kudonhedzera kwemusununguko kwaiva yekutanga immunohistochemical staining method.
Antigens inoonekwa apo inogadziriswa nemasikirini anoshandisa fluorescence dyes nekuda kwean antigen-antibody kusungirirana kushanda Izvi zvinowanikwa kana zvichisimbiswa nechiedza chinonakidza chechiedza chakasvibirira pasi pe microscope fluorescent.
I-osmolarity uye ion kuongororwa kwemasarudzo inofananidzwa nemuviri wemunhu-i isotonic.
A Recipe yePBS
Iwe unogona kugadzirira PBS nenzira dzinoverengeka. Kune mazita akawanda. Mamwe acho haana potassium, asi mamwe ane calcium kana magnesium .
Iyi kamukira iri nyore. Ndiyo 10X PBS stock solution (0.1M). Zvisinei, iwe unogonawo kugadzira 1X stock solution, kana kutanga neiyo recipe 10X uye uinatsire ku 1X.
Iyo yose inotora anenge maminitsi gumi.
Chimwe chisarudzo chekuwedzera Tween chinopiwawo.
Zvaunoda Kuti Uite PBS Buffer
- Sodium phosphate monobasic (anhydrous)
- Sodium phosphate dibasic (anhydrous)
- Sodium chloride
- Chiyero uye kuongorora zvikepe
- Magnetic stirrer uye simbisa bhari
- Chirwere chepH chinonyatsogadziriswa uye chakakodzera kugadzirisa kugadzirisa pH
- 1L kuvhuvhuta flask
- Pakati pe20 (kusarudza)
Nzira Yokuita Phosphate Buffered Saline (PBS)
Kurema 10,9g anhydrous sodium phosphate dibasic (Na2HPO4), 3.2g anhydrous sodium phosphate monobasic (NaH2PO4), uye 90g sodium chloride (NaCl).
Dissolve muzasi pasi pe1L mvura yakasvibiswa.
Ruramisa pH kusvika pane 7.4 uye ita mhinduro kusvika kumugumo wokupedzisira we1L.
Deredza 10X isati yashandiswa uye shandura pH kana zvichidikanwa.
Iwe unogona kuita PBS solution ine 0.5 muzana Tween 20 nekuwedzera 5mL Tween 20 kusvika 1L s solution.
Mazano Okuita PBS Buffer
Chengetedza chigadziro chiri kutonhorera mushure mekunge waita PBS sarudzo.
Zvisizvo zvisingagoneki zvinogadzirisa zvinogona kuchinjwa asi iwe unofanirwa kudzokorora mashekeri akaenzana ega ega kuti agone mvura yakawedzerwa yemamolekoro.
Zvishandiso zvePhosphate Buffered Saline (PBS)
Phosphate buffered saline ine kushandiswa kwakawanda nokuti iri isotonic uye isiri chepfu kune mamwe masero. Inogona kushandiswa kuderedza zvinhu uye inowanzoshandiswa kushambidza zvizere zvemasero. PBS inogona kushandiswa senzira inogadziriswa nenzira dzakasiyana-siyana yekuomesera biomolecules nokuti mvura yemamolekemu mukati mayo ichagadzirwa zvakapoteredza zvinhu-protein, somuenzaniso. Icha "eomeswa" uye haigadziriswi kusvika kune yakasimba.
Iko pH inoramba yakasimba uye ichienderana nekudzivirira kuparadzwa kwemasero.
Iyo firimu yakaonda yemvura inosungira kune iyo inodzivirira kudzivisa kana zvimwe zvinoshanduka kuchinja. Zvimedu zvinokonzerwa neCarbonate zvinogona kushandiswa nokuda kwechinangwa chimwechete asi kwete nekubudirira.
PBS inogonawo kushandiswa kutora kutaurwa inonzi spectrum painoyera mapuroteni adsorption mu ellipsometry.