Kune matanho akasiyana siyana anogona kushandiswa kufungidzira uye kuverenga DNA mushure mekunge zvinhu zvakaparadzana ne gel electrophoresis. Pakati pezvisarudzo zvakawanda, matambudziko mashanu aya anotanga kutangira neEthidium bromide, iyo ndiyo inoshandiswa zvikuru. Paunenge uchishanda nemugadziriro iyi, zvinokosha kuti urege kuziva chete kusiyana pakati pematambudziko asi hutano hwehutano.
01 Ethidium Bromide
Ethidium bromide ndiyo inenge yakave inozivikanwa zvikuru iyo inoshandiswa pakuona DNA. Dhayi iyi inogona kushandiswa mumusanganiswa wegelisi, mumusana we electrophoresis kana kuti kusvibisa gel mushure mekunge ichitanga. Makorekemu eDeyi anotevedzera DNA nemicheni uye fluoresce pasi pekuvhenekera kweVU, vachikuratidza chaizvo kuti mabhande ari mukati mejeri. Pasinei nezvakanakira, kukanganisa ndechokuti ethidiumdium bromide ndiyo inokonzerwa nekenzajeni, saka inofanira kutarisirwa nehanya zvikuru. Nemhaka yehutano hwehutano hwekushanda nejeri iyi, dzimwe nzira dzakawanikwa. 02 SYBR Ndarama
SYBR Dhayi yegoridhe inogona kushandiswa kuparadza DNA mbiri kana imwe isina kubatana, kana kuti kushatisa RNA. SYBR Ndarama yaiva imwe yenzira dzokutanga kune ethidium bromide yekurova pamusika uye inofungidzirwa kuti inonzwisisika kupfuura ye-ethidium bromide. Dhayi inoratidza 1000-guru kukurumidza fluorescence kana yaenda kune nucleic acids. Iyo inopinda yakareba uye yakakwirira yezana agarose gels uye inogona kushandiswa mu formaldehyde gels. Nokuti fluorescence ye molecule isina kuvhara yakanyanyisa, kushandura hazvidiwi. Chiremba-holder Molecular Probes (zvakare kubvira pakatanga yeSBR Gold) yakagadzirwa uye inotengeswa SYBR Safe uye SYBR Green ndiyo nzira dzakachengeteka kune ethidium bromide.
03 SYBR Green
Iyo SYBR Green I uye II tsvina (zvakare, inotengeswa neMorecular Probes) yakagadziriswa nenzira dzakasiyana. Nemhaka yokuti vanozvisunga kuDNA, ivo vachiri kungofungidzirwa kuti vanogona kutengesa uye nekuda kweizvozvo, vanofanira kutarisirwa nehanya. SYBR Green Ini ndinonyanya kunzwisiswa kuti ndishandiswe neDNA inopetwa kaviri, asi SYBR Green II, kune rumwe rutivi, inonyanya kushandiswa neDNA imwe chete kana kuti RNA. Kungofanana nedhizha rakakurumbira reEthidium Bromide, idzi idzi dzinotonhora zvakanyanya fluoresce pasi pekuvhenekera kweU UV. 04 SYBR Akachengeteka
SYBR Yakachengeteka yakagadzirirwa kuva imwe nzira yakachengeteka yeEthidium Bromide nedzimwe tsvina dze SYBR. Hazvirevi senzvimbo inoparadza uye inogona kuendeswa kuburikidza nemigwagwa yenguva dzose yemvura (kureva pasi pasi), nokuti kuongororwa kwehuwandu kunoratidza kuti hakuna dambudziko rinokonzera. Kuedza kunoratidzawo kuti pane zvishoma kana kuti hapana genotoxicity kuSyria Hamster Embryo (SHE) masero, lymphocytes evanhu, mouse lymphoma cells, uye mumuedzo we AMES. Iyo tsvina inogona kushandiswa ne-blue-light transilluminator iyo inokonzera kuderedza kuderedza DNA iri kuonekwa uye inopa huri nani huri nani hwekupedzisira. 05 Eva Green
Eva Green iri davi rinotonhorera rinowanikwa kuti ridzivise PCR zvishoma kudarika mamwe madhayi. Izvi zvinoita kuti zvive zvakakosha zvikuru pamashandisirwo akaita sehuwandu chaihwo-nguva yePCR. Ichowo chisarudzo chakanaka kana iwe uri kushandisa nheyo yakanyanyisa-ginganisa gels kuti uwane DNA. Inonyatsogadzikana pamatambo makuru uye ine fasi yakanyanya kwazvo fluorescence pachayo, asi ine fluorescent yakawanda kana yakasungwa kuDNA. Eva Green yakaratidzawo kuti yakanyanya kwazvo kana pasina cytotoxicity kana mutagenicity.