Nzira Yokusunungura-Dya Bhakitiriya Tsika (Lyophilization)

Kurapa, kana kuomeswa kwemafuro, ndiyo inyanzvi ye laboratory

Kusununguka-kuoma, kunonziwo lyophilization kana cryodicication, ndiyo nzira yekubvisa mvura kubva pane chimwe chigadzirwa mushure mekunge yakanyungudzwa (uye yobva yaiswa pasi pemubhedha) ichibvumira kuti mvura ichinje kubva kune yakasimba kusvika mumhepo, isina kupinda mumvura. Ice (kana zvimwe zvinokonzerwa nefriji) zvakabviswa kubva pane chigadzirwa kuburikidza nekugadzirisa kwekuisa pasi uye kuvhara maserolekisi emvura zvinodzingwa kuburikidza nekushandiswa kwekudhakwa.

The Basics of Lyophilization

Imwe yenzira dzakanakisisa dzekuchengetedza bhakitiriya, fungal, mbiriso kana imwe mikoko inokonzera utachiona kwenguva yakareba ndeyekushandisa kushandiswa kwekuisa-kuoma. Iyi nzira shomanana ye laboratory inogona kuitwa nechinzvimbo chipi nechipi chekutengesa chiripo-chakaoma chichachengetedza tsika yako yekuunganidza. Nokuti lyophilization ndiyo inonyanya kuoma uye inodhura fomu yekuoma, iyo inowanzochengetedzwa kune yakashata, zvinhu zvinotonhora zvisingakoshi. Zvinhu zvisingakonzereki nekuvhenekera zvinowanzova lyophilized kuitira kuti firiji yekuchengetedza isingakoshi.

Izvi zvinogona kutora nguva shomanana maawa matatu, uye kwenguva yakareba maawa makumi maviri nemana, kwete kusanganisira tsika yekuwedzera nguva.

Iro Nhanho-Nhanho Nzira yekurapa

1. Chokutanga, kukura unhu hweusiku husiku, kana usvina, we microorganism paLB kana imwe yakakodzera yegarti yakagadzirwa.

2. Zvadaro, gadzira sora yakaremara chikwata chemoto nekudzidzira motokari mberi kwemazuva, uye makapu (rubber stoppers) akaiswa pachena. Ita mapepa emapepa akadhindwa nekutaridzika kwechimiro mukati memabhati asati agadzirira autoclaving. Zvimwewo, shandisa mabhesi ane makapu akaitirwa sterility .

1. Wedzera 4 mL lyophilization buffer kudivha. Kana zvichidiwa, masero anogona kusimiswa achishandisa ganda rakaoma ganda.

2. Kurumidza kuendesa tsika yekusimudzirwa kune zvimedu zvakasvibiswa. Wedzera anenge 1.5 mL per vial. Chisimbiso nebhokisi rerubhu.

3. Kusunungura chimiro chechimiro mukati membiya nekuisa midziyo mu -20 degrees Celcius freezer.

1. Kana imwe nguva tsika dzakanyorova, gadzirai ganda rakaoma nekuiisa uye nekubvumira nguva yekushisa yakakodzera nekudziya mamiriro ekudzikama. Ita izvi maererano nemirayiridziro emagadzirirwo emhando yakasvibirira-yakaoma yakashandisa iwe.

2. Zvinyatso, uye aseptically, isai makapu akasununguka pamusoro pemiti, kuitira kuti mhepo inogona kupukunyuka panguva yekushisa-kuoma, uye isai mizinga muimba yakaoma-yakaoma. Isa chivharo kumakamuri maererano nemirairo yemugadziri.

3. Bvumira tsika yako nguva yekudzikisa zvachose (yakaoma). Izvi zvingatora kubva maawa mashomanana kusvika usiku humwe zvichienderana nehuwandu hwemuenzaniso wega wega uye kuti ndeapi masampu mauri.

4. Bvisa sampuli kubva muimba yemvura yakaoma uye yakaoma maererano nemirairidziro edzimiti uye pakarepo usimbise mitsara nemabhuruku erubhura, ipapo chifukidziro chinoputika.

5. Chengetedza lyophilized culture collection pakushambidzwa kwekamuri.

Miti Yaunoda

• Bvisa-dryer
• Nutrient kana imwe yakakodzera agar plates (uye incubator kuti ikure tsika)
• Glass rod
• Lyophilization buffer
• Iko-crimp-top vials ine rubber stoppers (uye munhu anokanganisa kushandisa mapiki)
• Freezer

<sub>Dheuteronomio:

SP Scientific. Nheyo Dzakasununguka Pakusvuta Kuchema. http://www.spscientific.com/freeze-drying-lyophilization-basics/</sub>