Kuyera Enzyme Kinetics
Sezvakangoita zvishoma, enzyme ndiyo mapuroteni anoshanda sevanokonzera mukuderedza kuwanda kwekushandiswa kwemhepo kuitira kuti zviitike kuti zviitike. Kushandisa simba ndiro simba rinotarisirwa kuti riwane "maitiro" kubva apo vazhinji vanoita zvisingaitike nekukurumidza zvikuru (kana kuitika zvachose) kana vachigadziriswa.
Inyenzi dzinobatsira kugadzirisa zvakananga mune imwe nzvimbo (inozivikanwa senzvimbo inoshandiswa) apo inogadziriswa inosunga uye inoita kuti iite zvigadzirwa. Substrates dzine nzvimbo dzinoshandiswa dzinonyanya kuzivikanwa chete kuitira kuti hapana zvimwe zvinhu zvinosunga kana kuti zviitike pane imwe nzvimbo. Kufanana nekivha uye kiyi, imwe enzyme inobatsira imwe chete chaiyo (zvimwe zvisiri izvo).
Basa rakanyatsoshandiswa rinoshandiswa mumapurotini okudzivirira nzira kuti aratidze chikamu chekunatswa. Zvakakosha kuziva chiyuniti ichi paunenge uchitenga ma enzymes kuti uratidze chaizvo kuti yakawanda yei enzyme yakachena uri kuwana.
Kana kuenzanisa enzyme kinetics (chiyero chekuita kweine enzyme ine substrate - kana pamusoro), basa rakananga rinotsanangurwa sehuwandu hwe substrate iyo enzyme inoshandura (zvinogadziriswa catalyzed), per mg protein muzvigadziro zve enzyme, imwe neimwe yenguva.
Muenzaniso wemabasa chaiwo ungave:
- Basa rakasarudzwa re enzyme iri kure rakayerwa pamamiriyoni 150 emoles / min / mg mushure mekucheneswa uye 800 umoles / min / mg, mushure mekucheneswa.
Basa rinonyanya kukosha chiyero chinokosha cheine enzyme tsvina. Kusiyana kwemaketeni eine enzyme yakachena inofanira kuva nemafungiro akafanana uye kunyange kuderedza sarudzo ye enzyme kakawanda ichave yakangofanana nemabasa ehutano kunyange zvazvo kuchave nekusiyana kwehutano hwemaitiro emabasa. Izvi ndechokuti pakuverenga basa rakananga, nhamba (zviyero / ml) uye denominator (mg / ml) inosangana zvakafanana.
Basa rakanyatsosiyana rakasiyana nemabasa, asi kuverenga kwebasa rakadaro rinoramba richienderana nebasa rinokosha. Izvi zvinoreva kuti izvo zvakataurwa zvakanakira basa rinozove zvakare zvichienderana nehurukuro yenhengo ye enzyme.
Mazhenje emakemikari ane zvishoma kudarika zvaatararisirwa kuita basa rinokosha angave ane enzyme makemikoro akave akashandurwa kana kusanganiswa nezvinhu zvisina kuchena.
Zvinokonzera Kuita Mabasa Enzyme
Zvinokwanisika kuti imwe enzyme inogona kunge yakaenzana nemaitiro emabasa ekuenzanisa kana ichienzaniswa nemarasi akasiyana (ndiko kuti, kupesana kwechokwadi mumabasa ekuenzanisa, kwete kusiyana kwakasiyana kunokonzerwa nokushandiswa kwezvakasiyana siyana tsanangudzo).
Nzira iyo chiyero chinoitwa (nzira yekuedza kuongorora kusachena kwemakemikari) ichaita kuti hutano hwemabasa hunozivikanwa. Somuenzaniso, enzyme inowanzoshanda mukati mekushisa kwe 37 degrees Celsius kana ichienzaniswa ne20 degrees Celsius, naizvozvo kana chiyero chichiitwa pa 21 degrees Celsius, hutsika huchasiyana nemasayiri akaitwa panopisa ye 32 degrees Celsius (kazhinji , zviyero zvinowanikwa panopisa pakati pe20-37 degrees Celsius).
Nokudaro, tsanangudzo yeiyo enzyme unit yaizove yakanyatsorondedzerwa se1 unit (U) inenge yakawanda ye enzyme inobatsira kuita 1 nmol ye substrate paminiti pasi pemamiriro ezvinhu akadai.