Sei E.coli Inoshandiswa kuGene Cloning

by Theresa Phillips

A Microorganism Inobatanidza Nezvinhu Zvinoshamisa

Inonzi microorganism Escherichia coli ine nhoroondo yakareba yekushandiswa mune zvigadzirwa zve biotechnology uye ichiri chidiki chezvinyorwa zvekusarudzwa kwemajini akawanda ekugadzirisa miedzo. Kunyange zvazvo E. coli inozivikanwa nehuwandu huwandu hwehutachiona hwehumwe hutambu hwakanyanya (0157: H7) vashomanana vanhu vanoziva kuti E-coli yakashandura uye inobatsira ndeyekutsvakurudza kwemafuro. Pane zvikonzero zvakawanda E. coli yakave yakashandiswa zvakanyanya uye ichiri inowanzogamuchirwa neDNA recombinant .

01 Genetic Simplicity

Mabhakitiriya anoita mashandisirwo anobatsira pakuongorora kwemajini nekuda kwehukuru hwavo hwemagenome eenzanisa neEukaryotes. E. coli masero ane mazana mana mana chete asi maitiro evanhu vegome anoona kuti vanhu vane mamiriyoni makumi matatu emagetsi. Uyewo, mabhakitiriya, kusanganisira E. coli , anorarama upenyu hwavo hwose muhuloid yenyika (ine bhokisi rimwe chete remakromosomes asina kubhadhara). Somugumisiro, hapana kechipiri chekromosomes kuti chifukidze migumisiro yezvigadziriswa mukati meproteinine engineering experiments.

02 Growth Rate

Mabhakiteriya anowanzokura nokukurumidza kudarika zvipembenene zvakaoma. E. coli inokura nokukurumidza pamwero wechizvarwa chimwe chete pamaminitsi makumi maviri pasi pekufananidzira kukura kwemamiriro. Izvi zvinobvumira kugadzirisa kwelogi-chikwata (pakati pemugwagwa kusvika kune huwandu hwemashoko) tsika pamusiku uye mazamu anoedza kuongororwa mumaawa mashoma pane mazuva mazhinji, mwedzi, kana makore. Kukurumidza kukura kunorevawo maitiro ekuvandudza maitiro apo tsika dzinoshandiswa mukugadzirisa-kupera kushandiswa.

03 Kuchengetedzwa

E. coli inowanzowanika mumatumba emukati evanhu nemhuka iyo inobatsira kupa mishonga (vitamini K neB12) kune iyo inopinda. Kune zvakawanda zvakasiyana siyana zveE E. coli dzinogona kubudisa chepfu kana kukonzera maitiro akasiyana-siyana ehutachiona kana inyirwa kana inobvumirwa kupinda mune dzimwe nhengo dzomuviri. Pasinei nembiri yakaipa yeimwe inonyanya kuipa (O157: H7), E. coli inowanzova isina mhosva kana ikashandiswa nehutano hwakachena.

04 The E. Coli Genome Zvakanaka Kunonzwisiswa

Nhengo yeE E. coli ndiyo yakatanga kuiswa zvakakwana (muna 1997). Somugumisiro, E. coli ndiyo inyanzvi yakawanda inodzidza michina. Nzwisisiro yepamusoro yeprotini inotaura nzira dzinoita kuti zvive nyore kushandisa kuongorori apo kuratidzwa kwemaproteni emunyika uye kusarudzwa kwemashure kunokosha.

05 Simba rekugara kune dzimwe nyika DNA

Nzira dzakawanda dzekugadzirisa mazita ejeni dzakagadzirwa kuburikidza nebhakitiriya uye zvichiri kubudirira kana kubudirira muna E. coli kupfuura mune dzimwe mishonga. Semugumisiro, kugadzirira kwemasero anokwanisa (masero anotora DNA dzisiri kune dzimwe nyika) haisi nyore. Kuchinjwa nemamwe machirangaridzo kazhinji hakusi kubudirira.

06 E Coli Inonyoresa Kutarisira

Nemhaka yokuti inokura zvakanyanya mumvura yevanhu, E. coli inoona zviri nyore kukura apo vanhu vanogona kushanda. Semuyenzaniso:

Inonyatsonakidzwa pakupisa kwemuviri. Kunyange zvazvo 98.6 degrees inogona kunge ichidziya kuvanhu vakawanda, zviri nyore kuchengetedza kuti kutonhora mune laboratori.
E. coli anogara mumatumbi evanhu, izvo zvinoreva kuti hazvisi kukakavara nezvezvokudya zvayo. Chaizvoizvo, zvinofadza kudya chero rudzi rwezvokudya zve predigested.
Inogona kukura zvose aerobically uye anaerobically. Nokudaro, inogona kuwedzera mumuviri wemunhu kana mhuka asi inofara zvakafanana mune petri ndiro kana flaski.

Sei E. Coli Anoita Kusiyana

E. Coli chigadzirwa chinoshamisa chezvinhu zvejini genetic; semugumisiro, yakatove yakagadzira mararamiro anoshamisa emishonga nemichina. Iyo ine kunyange, maererano neVanorikir Mechanics, inova mufananidzo wokutanga we bio-kombiyuta: "Mukushandurwa kweE E. coli" transcriptor, "yakagadzirwa nevatsvakurudzi veStanford University iyi yapfuura munaMarch, chikwata cheDNA chinomira nokuda kwetambo uye enzymes nokuda kwema electrons. Zvichida, iyi inhanho yekuvaka makombiyuta akashanda mumasero ehupenyu angakonzerwa kurongedza mazwi ekugadzira muhupenyu. " Kutsva kwakadaro kunogona kungoitwa nekushandiswa kwechimiro chinonyatsonzwisiswa, chiri nyore kushanda pamwe, uye chinokwanisa kupindura nokukurumidza.