- kutevera kana kutora mazita
- delivery
- scaffold / platforms
Mamwe nanoparticles ave achishandiswa kubva kuma1990, nokuda kwekushandiswa kwakafanana nekugadzirwa kwezvipfeko / zvekuchengetedza kweganda, kushandiswa kwezvinodhaka uye kuiswa mazita. Kuongorora nemhando dzakasiyana dzeanoparticles dzakadai sevotum dots, carbon nanotubes uye magnetic nanoparticles, pamasero e-somatic kana ma microorganisms, akapa ruzivo rwakabva kune tsvina yekutsvaga masero. Icho chinhu chinozivikanwa zvishomanana kuti yekutanga patent yekugadzirira kwemaofibers yakanyorwa muna 1934. Tsvina idzi dzaizopedzisira dzikazova nheyo dzezvigadziriswa zve stem cell culture uye kudhindwa-makore makumi manomwe gare gare.
Kuona maSero eStem Kushandisa MRI uye SPIO Particles
Kutsvakurudza pamusoro pekushandiswa kwei nanoparticles ye magnetic resonance imaging (MRI) yakakonzerwa nechido chekucherechedza stem cell therapeutics. Chisarudzo chinowanzodiwa chekushandiswa iyi ndeche superparamagnetic iron oxide (SPIO) nanoparticles, iyo inowedzera kusiyana kweMRI mifananidzo.
Mamwe massiksi esimbi akatotogamuchirwa neDFA. Mhando dzakasiyana dzezvikamu zvinoputirwa nemapurimi akasiyana pane kunze, kazhinji karubhaidhydrate. Kunyora kweMRI kunogona kuitwa nekubatanidza nanoparticles kusvika kune stem cell surface kana kukonzera kubatwa kwechikamu ne stem cell kuburikidza ne endocytosis kana phagocytosis.
Nanoparticles yakabatsira kuwedzera kuzivo redu nezvekuti masero emukati anotamira sei munheyo yekutya.
Kunyora Kushandisa Zvinyorwa Zvakawanda
Zvinyorwa zvitsvuku (Qdots) zvinokonzera zvikanda zvinokonzera chiedza uye zvinowanikwa maatomu kubva kumapoka II-VI etafura ye periodic, kazhinji inosanganisira cadmium. Ivo vari nani pakuona masero kune dzimwe nzira dzakadai sedhayi, nekuda kwekudzivirira kwavo uye kwenguva refu. Izvi zvinobvumirawo kushandiswa kwavo pakudzidza mashandisi emagetsi apo kusvibiswa kwemasero emukati ari kufambira mberi.
Mhedziso dzine shoma shoma rekushandisa nemasero emadziro kupfuura SPIO / MRI uye yakashandiswa chete mu vitro kusvika ikozvino, nekuda kwechidimbu chemichina chaiyo yekuzviona mumhuka dzose.
Nucleotide Zvokutumira Genetic Control
Genetic controls, kushandisa DNA kana siRNA , iri kubuda senzvimbo inobatsira yekudzora masero emasero mumasero emasero, zvikurukuru kutungamirira kusiyanisa kwavo. Nanoparticles inogona kushandiswa kushandura mitemo yakashandiswa nemagetsi, zvakadai se retroviruses, iyo yakave yakabatanidzwa mukukonzera matambudziko muzvisikwa zvipenyu zvakadai sokukonzera kushanduka kunoita kenza. Nanoparticles inopa mari shoma, inonyanya kuwanikwa vector yekuwedzerwa kwemasero ematsipa, ine njodzi yakaderera yekudzivirira muviri, kusagadzikana kana kuora.
Nzira yakakurumbira ndeyokushandisa cationic mapurogiramu anobatana neDNA uye RNA molecules. Panewo nzvimbo yekusimbiswa kwemapurimi ekudzidzira , ane zvinhu zvakadai sekuendeswa kwekutora kana kurongwa kusunungurwa . Carbon nanotubes nemapoka akasiyana-siyana akave akaongororwa nezvemishonga uye nucleic acid ekuberekerwa mumasero emammalian, asi kushandiswa kwavo mumasero emukati hauna kuongororwa zvikuru.
Kugadzirisa Semu Cell Cell Environment
Chinhu chakakosha chekudzidza mu stem cell tsvakurudzo ndeyeyedzimwe nzvimbo yakashambadzirira uye kuti zvingave sei kunze kwesero zvinotumira zviratidzo zvekutonga kwekusiyanisa, kuenda kune dzimwe nyika, kubata pamwe nezvimwe zvinhu. The extracellular matrix (ECM) , inoumbwa nemakemikisi yakavanzwa nemasero akafanana nekorgen, elastin, uye proteoglycan. Izvo zvezvinhu izvi zvinokonzerwa nemasikirwo uye makemikari ezvakatipoteredza zvavanenge vasika, inopa nhungamiro ye stem cell mabasa.
Nanoparticles dzave dzichishandiswa pakugadzira zvakasiyana-siyana maitiro ezvinyorwa zvinoratidza maECM, nekuda kwezvavakaita pamasero emadziro.
Chirwere chikuru chakasangana ne stem cell therapies chakanga chiri kusakwanisa kwemasero akajowa kuti azvinyorwe kuti atore mishonga. Nanoscale inowedzera kuvandudza masero ekupona kuburikidza nekubatsira kuburitsa. Nanofibers inoshandiswa kubva pakugadzira mapurisa akadai se poly (lactic acid) (PLA), kana mapurogiramu chaiwo e collagen, mapuroteni esiriki kana chitosan, anopa nzira dzekugadziriswa kwemasero nemagetsi. Chinangwa chikuru ndechekuona kuti zvakakosha zvakakosha zvinokurudzira sei zvakanamatira uye kuwedzera kwemasero emadziro uye kushandisa shanduro iyi ye stem cell transplantations. Zvisinei, zvinoratidzika kuti morphology yemasero akakurira pane nanofibers inogona kusiyana nemasero akakurira kune mamwe mhizha, uye zvishomanana muzvivo zve vivo zvataurwa.
Nanoparticle Toxicity kuStem Cells
Sezvo nezvose zvinowanikwa mumagariro ezvinyorwa, kushandiswa kwe nanoparticles yezvikumbiro izvi mu vivo (mumunhu) zvinoda kubvumirwa kweDFA. Nekuwanikwa kwekwanisi ye nanoparticles ye stem cell applications, yasvika kuwedzera kwekuda kwekuedza kwekliniki kuedza kutsvaga kutsva uye kuwedzerwa kufarira mune nanoparticle toxicity .
Icho chinotyisa cheSSPIO nanoparticles chakadzidzwa zvikuru. Pamusoro pezvikamu zvizhinji, hazvina kuonekwa zvakasviba, asi kumwe kuongorora kwakaratidza kuchinja kwekusiyanisa kwemasero emadzimai. Kunyange zvakadaro, pane chimwe chikonzero chekusava nechokwadi chekuti chepfu inokonzerwa ne nanoparticles kana kuti inopihwa mutengesi / mutezo.
Dhiyabhorosi dhiyabhorosi yeQdots inoshaikwa, asi chii chinyorwa chiripo havasi vose vanobvumirana. Zvimwe zvidzidzo zvinorondedzera pasina migumisiro yakaipa pa stem cell morphology, kupararira, nekusiyanisa, asi vamwe vanorondedzera kusakanganisika. Kusiyana kwemigumisiro yemuedzo kunogona kuonekwa kune zvakasiyana-siyana zvei nanoparticles kana kuti zvinoshandiswa masero, saka zvakanyanya kutsvakurudza zvinodiwa kuti zvive izvo zvakachengeteka uye zvisingaiti, uye kuti ndeapi mapoka emasero. Chinozivikanwa ndechekuti cadmium inokonzerwa (Cd2 +) inogona kuva yakasviba nemhaka yemigumisiro yayo pane mitochondria yemasero. Izvi zvinowedzera zvakaoma nekubudiswa kwemaitiro anoshandiswa e oxygen munguva yeCdd degradation.
Carbon nanotubes inowanzove inowanzoitwa genotoxic, zvichienderana nemafungiro avo, mashizha, mafungiro uye kuumbwa kwenyika, uye zvinogona kuita kuti kuve kwemajini anogadziriswa mumasero.
Nanoparticles vari kuvimbisa zvishandiso zvemaitiro matsva emagetsi, nekuda kwekukura kwavo uye simba ravo kupinda mukati memasero. Sezvo kutsvakurudza mberi kunoenderera mberi kuwedzera kune zivo yedu yezvinhu zvinodzora stem cell function, zvinogona kunge zvitsva zvinoshandiswa zve nanoparticles, pamwe chete nemasero emadzimai, zvichawanikwa. Kunyange zvazvo uchapupu hunoratidza kuti mamwe maitiro anozoita kuti ave anobatsira, kana kuti akachengeteka, kupfuura mamwe, pane mikana yakawanda yekushandisa nanoparticles kusimbisa nekuvandudza stem cell technologies.
> Kwakabva:
> Ferreira, L. et al. 2008. Mikana mitsva: Kushandiswa kweanotechnologies kushandura uye kutarisa masero emadzimai. Cell Cell Stem 3: 136-146. doi: 10.1016 / j.stem.2008.07.020.